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Algal Growth Media

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Algal Growth Media

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Quick Overview

Algal growth media are nutrient-enriched liquid solutions that support the growth of algae. Periodic dilution of actively growing algal cultures with new growth medium can sustain the culture indefinitely. The seawater base is collected from Half Moon Bay (California), ultraviolet germicidal irradiated, enriched with 2 macro- and 14 micro-nutrients, and the final formulation is sterile filtered (no autoclaving or heat) to ensure quality and extended shelf life. Growth media are packaged in quantities of 500 mL (16.9 fl oz) in Nalgene™ PET sterile square bottles.

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Growth media extend the lifespan or increase the volume of an existing algal culture.

L1 medium is recommended for the culture of bioluminescent algae. L1 is an updated formulation of Guillard's f/2 medium. The BMC cultures all bioluminescent algae on L1 medium.

Empco L1 Medium Technical Specifications:
The L1-Si (L1 minus Silica) enriched seawater medium is an updated formulation based on the f/2 medium (Guillard and Ryther 1962). L1-Si contains additional trace metals compared to f/2, and omits silica to reduce precipitation. It is a general purpose liquid marine medium for growth of coastal algae. Empco's L1 medium is a full-strength seawater medium (34 ppt) prepared at 2X nutrient concentration of the original L1 formulation to enhance growth of dinoflagellates, and should be used undiluted. This medium may be used to renew actively growing cultures of bioluminescent algae. To use, combine approximately 1 part algal culture with 3 to 5 parts growth medium, or as needed. Detailed instructions are included with purchase.